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Liquid Chromatography (LC)
Liquid Chromatography (LC)
Chromatography involves separating a mixture by passing it through a separation medium that gradually traps solutes due to their size – in liquid chromatography (LC) a sample is dissolved in suitable solvent and passed through a separation medium as a liquid. This could be done immediately ahead of analysis step, perhaps to see how much of a specific protein is within a sample, or it could be done to synthesise a highly purified material for later fabrication steps. LC is commonly performed at high pressure (high-performance liquid chromatography (HPLC)), which his able to resolve to a higher degree, or in conjunction with a mass spectrometer (LC-MS) for immediate analysis.
List of available equipment
TOOL MAKE AND MODEL
KEY DIFFERENTIATOR
LOCATION
Agilent Technologies 1260 Infinity_2
High performance liquid chromatography (HPLC) system
Optofab Node
University of Adelaide
Description
Identify, and quantify components in a liquid mixture
Related Information
Key features: This HPLC is fitted with an automatic sampler; the detection mode is based on UV-Vis absorption; the approximate wavelengths are between 215 - 360 nm.
Tool Contact
optofab@adelaide.edu.au
Agilent Technologies 1260 Infinity
High performance liquid chromatography (HPLC) system
Materials Node
University of Wollongong
Description
HPLC system for materials separation and characterisation
Related Information
Organic HPLC System
Tool Contact
innis@uow.edu.au
Gilson GX-271
Semi-preparative HPLC
Optofab Node
University of Adelaide
Description
Unit has automated sampling and fraction collection functions.
Related Information
Capacity per run is approximately less than 10 mg/mL of sample.
Tool Contact
optofab@adelaide.edu.au
Shimadzu LC20 – Gel Permeation Chromatography System HPLC LC20 GPC
HPLC - specialist GPC system (aqueous & non-aqueous)
Materials Node
University of Wollongong
Description
HPLC system - specialist GPC system (aqueous & non-aqueous)
Related Information
Optimised for Gel Permeation Chromatography - polymer molecular weight determination.
Tool Contact
innis@uow.edu.au
Shimadzu LCMS 2020 2020-LCMS
Liquid chromatography mass spectrometry (LC-MS) system
Materials Node
University of Wollongong
Description
Liquid Chromatography Mass Spectrometer for materials separation and characterisation
Related Information
Organic HPLC System
Tool Contact
innis@uow.edu.au
Waters Alliance 2695 Separations Module
Liquid chromatography mass spectrometry (LC-MS) system
QLD Node
University of Queensland
Description
Technique for the separation and detection of compounds using various mobile phases with a UV detector coupled with a mass spectrometer.
Related Information
Micromass Quattro micro API. LC-MS range of 20-2000 Da. Standard C18 column in use. Can be used for the separation of pharmaceutical and biopharmaceutical samples, proteins, peptides for further analysis. Columns separate samples based on ionic, aqueous and organic based environment.
Tool Contact
anff@uq.edu.au
TOOL MAKE AND MODEL
KEY DIFFERENTIATOR
LOCATION
Agilent Technologies 1260 Infinity_2
High performance liquid chromatography (HPLC) system
QLD Node
University of Queensland
Description
Identify, and quantify components in a liquid mixture
Related Information
Key features: This HPLC is fitted with an automatic sampler; the detection mode is based on UV-Vis absorption; the approximate wavelengths are between 215 - 360 nm.
Tool Contact
optofab@adelaide.edu.au
TOOL MAKE AND MODEL
KEY DIFFERENTIATOR
LOCATION
Agilent Technologies 1260 Infinity
High performance liquid chromatography (HPLC) system
QLD Node
University of Queensland
Description
HPLC system for materials separation and characterisation
Related Information
Organic HPLC System
Tool Contact
innis@uow.edu.au
TOOL MAKE AND MODEL
KEY DIFFERENTIATOR
LOCATION
Gilson GX-271
Semi-preparative HPLC
QLD Node
University of Queensland
Description
Unit has automated sampling and fraction collection functions.
Related Information
Capacity per run is approximately less than 10 mg/mL of sample.
Tool Contact
optofab@adelaide.edu.au
TOOL MAKE AND MODEL
KEY DIFFERENTIATOR
LOCATION
Shimadzu LC20 – Gel Permeation Chromatography System HPLC LC20 GPC
HPLC - specialist GPC system (aqueous & non-aqueous)
QLD Node
University of Queensland
Description
HPLC system - specialist GPC system (aqueous & non-aqueous)
Related Information
Optimised for Gel Permeation Chromatography - polymer molecular weight determination.
Tool Contact
innis@uow.edu.au
TOOL MAKE AND MODEL
KEY DIFFERENTIATOR
LOCATION
Shimadzu LCMS 2020 2020-LCMS
Liquid chromatography mass spectrometry (LC-MS) system
QLD Node
University of Queensland
Description
Liquid Chromatography Mass Spectrometer for materials separation and characterisation
Related Information
Organic HPLC System
Tool Contact
innis@uow.edu.au
TOOL MAKE AND MODEL
KEY DIFFERENTIATOR
LOCATION
Waters Alliance 2695 Separations Module
Liquid chromatography mass spectrometry (LC-MS) system
QLD Node
University of Queensland
Description
Technique for the separation and detection of compounds using various mobile phases with a UV detector coupled with a mass spectrometer.
Related Information
Micromass Quattro micro API. LC-MS range of 20-2000 Da. Standard C18 column in use. Can be used for the separation of pharmaceutical and biopharmaceutical samples, proteins, peptides for further analysis. Columns separate samples based on ionic, aqueous and organic based environment.
Tool Contact
anff@uq.edu.au
